Résultats de la recherche - 16 results
Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV.
Publication Type: Peer reviewed scientific article Authors: Dejan Vidanović; Tešović, Bojana; Milanko Šekler; Zoran Debeljak; Nikola Vasković; Kazimir Matović; Andrey Koltsov; Kiril Krstevski; Tamaš Petrović; Ilse De Leeuw; Andy Haegeman Source: Microorga ...
Challenges to Differentiate Hepatitis C Genotype 1 and 6: Results from A Field-Study in Cambodia.
LightPower Taqman real-time PCR method (Viet A Corp.) and quality controlled using the Versant 2.0 line probe assay (Siemens Healthcare). Next, following interim quality control results, all 106 samples were ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika (ZIKV) ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.
transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, were selected from ...
Complete coding sequence of a novel picorna-like virus in a blackbird infected with Usutu virus.
consists of at least 9,228 nt and contains two open reading frames (ORF s) encoding the nonstructural polyprotein (2235 amino acids) and structural polyprotein (769 amino acids). Two TaqMan RT-qPCR assays ...
Development and validation of a TaqMan probe-based real-time PCR method for the differentiation of wild type lumpy skin disease virus from vaccine virus strains.
Publication Type: Peer reviewed scientific article Authors: Agianniotaki, Eirini I; Chaintoutis, Serafeim C; Andy Haegeman; Tasioudi, Konstantia E; Ilse De Leeuw; Katsoulos, Panagiotis-Dimitrios; Sachpatzidis, Achilleas; Kris De Clercq; Alexandropoulos, T ...
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.
ten avian gene primer and probe sets that can be used to identify suitable reference genes in many experimental contexts. The panel was tested with TaqMan and SYBR Green systems in two experimental ...
Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.
approach. A TaqMan® qPCR method was developed and tested on isolated bacterial strains and on the feed additive directly. RESULTS: In this study, a very simple strategy based on the common BLAST tools that ...
Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.
a TaqMan(®) probe specific for ASFV were selected from conserved regions of the p72 gene. The limit of detection of the new real-time PCR assay is 5.7-57 copies of the ASFV genome. High accuracy, ...
Rapport annuel 2010-2011 du GMOlab concernant les dossiers de validation pour l'identification des organismes génétiquement modifiés
Sciacqua,M.; Anne-Marie Vanherle; Draguet,V. Source: WIV-ISP; PBB, Brussels, Belgium, p.13 (2011) ISBN: D/2011/2505/32 Keywords: de GMOlab LE OGM identification qPCR rapport rapport annuel TaqMan(r) VALIDATION ...