Résultats de la recherche - 36 results
EFSA Project on the use of New Approach Methodologies (NAMs) for the hazard assessment of nanofibres. Lot 1, nanocellulose oral exposure: gastrointestinal digestion, nanofibres uptake and local effects
Fessard, Valerie; Jan Mast; Blanquet‐Diot, Stephanie; Bremer‐Hoffmann, Susanne; Cubadda, Francesco Source: EFSA Supporting Publications, Volume 20, Issue 9 (2023) Keywords: barrier crossing cell uptake ...
Novel prediction models for genotoxicity based on biomarker genes in human HepaRG™ cells.
DNA microarray profiles of 24 (non-)genotoxic reference chemicals covering different modes of action in metabolically competent human HepaRG™ cells. In the present study, new prediction models for ...
The GENOMARK transcriptomic biomarker demonstrates a high predictivity for genotoxic hazards and utility in potency ranking in human HepaRGTM cells
Publication Type: Scientific poster, presentation or proceeding Authors: Anouck Thienpont; Eunnara Cho; Andrew Williams; Matthew Meier; Carole Yauk; Vera Rogiers; Tamara Vanhaecke; Birgit Mertens Source: EEMGS meeting, Malaga, Spain (2023) Keywords: bench ...
The GENOMARK biomarker inhuman HepaRGTM cells: a new approach methodology for genotoxicity assessment
Publication Type: Scientific poster, presentation or proceeding Authors: Anouck Thienpont; Stefaan Verhulst; Leo van Grunsven; Vera Rogiers; Tamara Vanhaecke; Birgit Mertens Source: SaferWorldByDesign webinar, Online (2023) Keywords: genotoxicity NAM tran ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
the STEC strain from the endogenous E.coli strains while an Illumina MiSeq run takes 48 hours. Moreover, the lower-cost Flongle flow cell showed the same results after 24 hours of sequencing when using ...
A Multifaceted Approach for Evaluating Hepatitis E Virus Infectivity In Vitro: Cell Culture and Innovative Molecular Methods for Integrity Assessment
Publication Type: Peer reviewed scientific article Authors: Tatjana Locus; Lambrecht, Ellen; Sophie Lamoral; Willems, Sjarlotte; Steven Van Gucht; Vanwolleghem, Thomas; Michael Peeters Source: Veterinary Sciences, Volume 10, Issue 12 (2023) Health Topics: ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
evaluated the performances of 6 DNA extraction methods using various cells lysis and purification techniques, from quick and easy, to more time-consuming and gentle protocols, including a portable method for ...
Development, Validation and Application of a Targeted LC-MS Method for Quantification of Microcystins and Nodularin: Towards a Better Characterization of Drinking Water
(PPIIA), disrupting cell growth and metabolism [15,16]. When ingested, these toxins are transported by the bile salts to the liver, potentially causing liver damage [17,18,19]. Health Topics: Food ...
Genome Editing in Agriculture: Methods, Applications, and Governance—A paper in the series on The Need for Agricultural Innovation to Sustainably Feed the World by 2050
(CAST), Volume 60, Issue 60 (2018) Abstract: Genome editing is the process of making precise, targeted sequence changes in the deoxyribonucleic acid of living cells and organisms. Recent advances have made ...
A novel genotoxin-specific qPCR array based on the metabolically competent human HepaRG™ cell line as a rapid and reliable tool for improved in vitro hazard assessment
for pharmaceutical, chemical, and consumer products, as afflicted compounds have to be (prematurely) abandoned or further tested on animals. Using the metabolically competent human HepaRG ™ cell line and toxicogenomics ...