The first International Standard anti-Brucella melitensis Serum.

Last updated on 23-8-2019 by Anonymous (non vérifié)

Peer reviewed scientific article



The World Organisation for Animal Health (OIE) requested an International Standard anti-Brucella melitensis Serum (ISaBmS) to standardise diagnostic tests and reagents for sheep and goats. The agreed criteria were the highest dilution (in negative serum) of the standard which must give a positive result and the lowest dilution (in negative serum) which must simultaneously give a negative result. The two dilutions for each assay were, respectively: indirect enzyme-linked immunosorbent assay (iELISA) ⅙4 and 1/750, competitive ELISA (cELISA) ⅛ and ⅓00, fluorescent polarisation assay (FPA

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