Glucocorticoid-inducible gene expression vectors for use in Drosophila melanogaster Schneider 2 cells4410

Last updated on 22-8-2019 by Anonymous (non vérifié)

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Résumé:

Inducible, vector-based, expression systems that allow fine control of transgene expression are gaining more and more use in fundamental research as well as in therapeutic applications. In an effort to develop a tightly regulated heterologous expression system for Drosophila Schneider 2 cells, three different inducible reporter constructs were compared. These comprised six copies of the glucocorticoid response element fused to one of three distinct types of Drosophila gene promoters: (1) a TATA-box containing, (2) a TATA-less and (3) a bidirectional core sequence. These were fused to a luci…

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