Proof-of-concept study for successful inter-laboratory comparison of MLVA results

Last updated on 23-8-2019 by Anonymous (non vérifié)

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Published

Peer reviewed scientific article

SCIENSANO

Résumé:

Multiple-locus variable-number of tandem repeats analysis (MLVA) is widely used for typing of pathogens. Methods such as MLVA based on determining DNA fragment size by the use of capillary electrophoresis have an inherent problem as a considerable offset between measured and real (sequenced) lengths is commonly observed. This discrepancy arises from variation within the laboratory set-up used for fragment analysis. To obtain comparable results between laboratories using different set-ups, some form of calibration is a necessity. A simple approach is to use a set of calibration strains with …

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