Last updated on 23-8-2019 by Anonymous (non vérifié)
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Auteurs
Supply,P.; Allix,C.; Lesjean,S.; Cardoso-Oelemann,M.; Rusch-Gerdes,S.; Willery,E.; Savine,E.; de Haas,P.; van Deutekom,H.; Roring,S.; Bifani,P.; Kurepina,N.; B.N. Kreiswirth; Sola,C.; Rastogi,N.; Vatin,V.; M.C. Gutierrez; M. Fauville; Niemann,S.; R.A. Skuce; K. Kremer; Locht,C.; D. van SoolingenMots-clés
Résumé:
Molecular typing based on 12 loci containing variable numbers of tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTRs) has been adopted in combination with spoligotyping as the basis for large-scale, high-throughput genotyping of Mycobacterium tuberculosis. However, even the combination of these two methods is still less discriminatory than IS6110 fingerprinting. Here, we define an optimized set of MIRU-VNTR loci with a significantly higher discriminatory power. The resolution and the stability/robustness of 29 loci were analyzed, using a total of 824 tubercle bacillus …