Validation of a Targeted LC-MS/MS Method for Cereulide and Application in Food and Faeces.

Last updated on 29-1-2024 by Julien Masquelier

Peer reviewed scientific article




Cereulide is an emetic toxin produced by some strains of . This bacterial toxin, a cyclic 1.2 kDa dodecadepsipeptide, is stable to heat and acids and causes nausea and vomiting when ingested via contaminated food. This work aimed to develop and validate a targeted analytical method applying liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify this toxin in food and human faeces. Samples were extracted with acetonitrile in the presence of C-cereulide, a labelled internal standard, and purified by centrifugation and filtration. The limits of quantification were 0.5 and 0.3 µg…

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