Résultats de la recherche - 31 results
Towards facilitated interpretation of shotgun metagenomics long-read sequencing data analyzed with KMA for the detection of bacterial pathogens and their antimicrobial resistance genes
Michelacci, Valeria; Matamoros, Bosco Rodríguez; González-Zorn, Bruno; Brouwer, Michael S. M.; Di Marcantonio, Lisa; Bloemen, Bram; Vanneste, Kevin; Roosens, Nancy H. C. J.; AbuOun, Manal; De Keersmaecker, ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
laboratories in the future. The method was first tested on minced beef spiked with shiga toxin-producing E.coli (STEC) at very low level (5 CFU /25g). After enrichment for 16 or 24 hours in buffered peptone ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
Publication Type: Peer reviewed scientific article Authors: Mathieu Gand; Bram Bloemen; Kevin Vanneste; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: BMC Genomics, Volume 24, Issue 1 (2023) ...
Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products
Publication Type: Peer reviewed scientific article Authors: Fraiture, Marie-Alice; Gobbo, Andrea; N. Papazova; Roosens, Nancy H. C. Source: Fermentation, Volume 9, Issue 11 (2023) Health Topics: ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
Sigrid C.J. De Keersmaecker; Kevin Vanneste; Nancy Roosens Source: Foods, Volume 12, Issue 3 (2023) Keywords: CRISPR/Cas9 detection food and feed chain Genetically modified organism high-throughput ...
Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products
Publication Type: Peer reviewed scientific article Authors: Marie-Alice Fraiture; Andrea Gobbo; N. Papazova; Nancy Roosens Source: Fermentation, Volume 8, Issue 2 (2022) Keywords: additives and ...
Development, evaluation and optimization of next generation sequencing data analysis tools in support of a fast response for public health and food chain safety
Publication Type: Dissertation Authors: Assia Saltykova Source: Ghent University, Ghent, Belgium, p.308 (2022) Keywords: bioinformatics tools Database GMO Metagenomics NGS outbreak investigation ...
Listeria monocytogenes isolates from Cornu aspersum snails: Whole genome-based characterization and host-pathogen interactions in a snail infection model.
virulence, and host-pathogen mechanisms of interactions in a snail infection model. Most of isolates (66.7%) were characterized as multidrug resistant (MDR) and belonged to clonal complexes (CC s) which are ...
Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database
Publication Type: Peer reviewed scientific article Authors: Assia Saltykova; Julien Van Braekel; N. Papazova; Marie-Alice Fraiture; Deforce, Dieter; Kevin Vanneste; Sigrid C.J. De Keersmaecker; ...
Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a Salmonella food-borne outbreak
strain-level shotgun metagenomics approach for source tracking. We received two suspect food samples: the full meal and some freshly made tartar sauce served with this meal, requiring the use of raw eggs. After ...