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Zona pellucida gene mRNA expression in human oocytes is related to oocyte maturity, zona inner layer retardance and fertilization competence.
analysed. Oocytes were examined using PLM, and quantitative RT- PCR was performed for ZP1, 2, 3 and 4 in these individual oocytes and their CC. Ephrin- B2 (EFNB2) mRNA was measured in CC as a control. ...