Résultats de la recherche - 35 results
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
adaptive sampling, following whole genome amplification to increase the amount of genetic material to meet sequencing standards. The strain-level data analysis was performed as previously described (Buytaers ...
Occurrence of microcystin congeners in surface waters and food in Belgium as a source of exposure to humans
the producers of these toxins by amplification and sequencing of the mcyE gene. Yet, data on the MC s prevalence is not sufficient to accurately assess human exposure and risk. Additional consumption ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
preparation methods that could enhance the detection of the virus in the sample or during the sequencing (i.e. adaptive sampling). Overall, we showed that shotgun metagenomics, with or without amplification ...
Redesign and Validation of a Real-Time RT-PCR to Improve Surveillance for Avian Influenza Viruses of the H9 Subtype.
for molecular-based diagnostic methods and was the cause for suboptimal detection and false negatives during routine diagnostic monitoring. Starting from a dataset of sequences related to viruses of ...
A Summer of Cyanobacterial Blooms in Belgian Waterbodies: Microcystin Quantification and Molecular Characterizations
method allowed to detect and quantify eight microcystin congeners. The mcyE gene was detected using PCR, while dominant cyanobacterial species were identified using 16S RNA amplification and direct ...
Development of a genoserotyping system for the identification of Salmonella serotypes
detection of molecular markers or on the analysis of sequencing data, are described in the scientific literature. Based on a critical review of some of these techniques, the Multiplex Oligonucleotide ...
Strategy for the identification of micro-organisms producing food and feed products: Bacteria producing food enzymes as study case
amplification and sequencing of the 16S-rRNA gene, the potential presence of FE producing bacteria in FE preparations. First, the specificity was verified using all microbial species reported to produce FE. ...
A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
p.e0164463 (2016) Keywords: Animals DNA Primers Foot-and-Mouth Disease Virus Humans Real-Time Polymerase Chain Reaction Abstract: Non-specific tail sequences are often added to the 5’-terminus of primers to ...
Next-generation sequencing workflows in veterinary infection biology: towards validation and quality assurance.
High-Throughput Nucleotide Sequencing Nucleic Acid Amplification Techniques Quality Control Reproducibility of Results Workflow Abstract: Recent advancements in DNA sequencing methodologies and sequence data ...
Optimized MOL-PCR for Characterization of Microbial Pathogens
different types of markers, like unique sequences, single-nucleotide polymorphisms (SNP s) and indels, can be simultaneously analyzed in one assay. This assay consists of a multiplex ligation for detection of ...