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Résultats de la recherche - 28 results
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
a proof-of-concept, we explored the use of adaptive sampling using various databases (depletion of the food matrix i.e. potato, enrichment of a selection of foodborne pathogens, enrichment of a database of ...
Applicability of the DPRA on mixture testing: challenges and opportunities.
depletion of a sensitizer. In conclusion, we confirmed that the DPRA test method can be used efficiently for well-known characterized mixtures. However, when deviating from the recommended testing ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
water, the DNA was extracted with two classical commercial kits or with a kit performing depletion of the host DNA. The extracted DNA also was amplified or not using Phi29 polymerase. We showed that all ...
Decontamination process for dioxins and dioxin‐like PCBs from fish oil and vegetable oils and fats by a physical process with activated carbon
detrimental changes in the nutritional composition of the fish‐ and vegetable oils, it is possible that the process could deplete some beneficial constituents (e.g. vitamins). Taken together, it was not ...
Carry-over of some Fusarium mycotoxins in tissues and eggs of chickens fed experimentally mycotoxin-contaminated diets
μg/kg levels of HT-2 toxin (HT-2), T-2 toxin (T-2) and zearalenone (ZEN) during a repletion period of two weeks, followed by a depletion period of two weeks. DON, ZEN, T-2 and HT-2 were not carried out ...
Agglomeration of titanium dioxide nanoparticles increases toxicological responses in vitro and in vivo
TiO2 induced stronger responses than small agglomerates for glutathione depletion, IL-8 and IL-1β increase, and DNA damage in THP-1, while no effect of agglomeration was observed with 117 nm TiO2. In ...
Y-box-binding protein 1 supports the early and late steps of HIV replication.
replication. YB-1 depletion resulted in a 10-fold decrease in HIV-1 replication in different cell lines. Dissection of the replication defects revealed that knockdown of YB-1 is associated with a 2- to 5-fold ...
An application of mass spectrometry for quality control of biologicals: Highly sensitive profiling of plasma residuals in human plasma-derived immunoglobulin.
fractionation for this purpose. Sample fractionation proved mandatory to enable identification of plasma residuals. Two approaches were compared: Ig depletion with protein G — protein A affinity chromatography ...
High coverage metabolomics analysis reveals phage-specific alterations to Pseudomonas aeruginosa physiology during infection.
metabolism. Furthermore, clear phage-specific and infection stage-specific responses are observed, ranging from extreme metabolite depletion (for example, phage YuA) to complete reorganization of the metabolism ...
Evaluation of convenient pretreatment protocols for RNA virus metagenomics in serum and tissue samples.
removal nearly doubled once more the proportion of viral sequences. For lung tissue, a ribosomal RNA depletion step on the RNA extract had the biggest gain in proportion of viral sequences (32×). We show ...