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Résultats de la recherche - 18 results

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

Sigrid C.J. De Keersmaecker; Kevin Vanneste; Nancy Roosens Source: Foods, Volume 12, Issue 3 (2023) Keywords: CRISPR/Cas9 detection food and feed chain Genetically modified organism high-throughput ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

flavorings Bacillus subtilis group Enzymes food and feed safety concerns real-time PCR detection Unauthorized genetically modified microorganisms Abstract: Most of the bacteria that are used to produce ...

Listeria monocytogenes isolates from Cornu aspersum snails: Whole genome-based characterization and host-pathogen interactions in a snail infection model.

expression led only to an increase of necrotic non-apoptotic cells. Overall, this study provides significant insights into the genetic diversity of L. monocytogenes from snails, the genomic features of them ...

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

Microbiology, Volume 354 (2021) Keywords: Antimicrobial resistance genes DNA walking anchored on pUB110 food and feed safety Genetically modified bacteria Protease real-time PCR detection Abstract: A PCR-based ...

Adjunct Culture of Non-Starter Lactic Acid Bacteria for the Production of Provola Dei Nebrodi PDO Cheese: In Vitro Screening and Pilot-Scale Cheese-Making.

were genetically identified by Rep- PCR genomic fingerprinting, using the (GTG)-primer, and by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI- TOF MS). Twenty-seven ...

A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products

Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Food Chemistry: Molecular Sciences, Volume 2 (2021) Keywords: AMR genetically modified microorganism identification long and short read sequencing ...

Adjunct Culture of Non-Starter Lactic Acid Bacteria for the Production of Provola Dei Nebrodi PDO Cheese: In Vitro Screening and Pilot-Scale Cheese-Making

production. One hundred and twenty-one lactic acid bacteria, isolated from traditional Provola dei Nebrodi cheese samples, were genetically identified by Rep- PCR genomic fingerprinting, using the ...

Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products

resistance gene Food and feed microbial fermentation products Genetically modified microorganisms PCR-Based detection Abstract: Genetically modified microorganisms (GMM), harbouring commonly antimicrobial ...

How can we better detect unauthorized GMO in the food and feed chain

unauthorized genetically modified organisms (GMO s). Here, we propose a new workflow, based on next-generation sequencing (NGS) technology, to overcome this problem. In providing information about DNA sequences, ...

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