Résultats de la recherche - 68 results
Metagenomics-based tracing of genetically modified microorganism contaminations in commercial fermentation products
frequently employed for the production of microbial fermen- tation products such as food enzymes. Although presence of the GMM or its recombinant DNA in the final product is not authorized, contaminations ...
be.Prepared: Setting up a cloud-based bioinformatics platform and coupled NRC usability platform for integrated genomic-epidemiological analysis at a national level
Azure cloud. It processes pseudonymized microbial WGS data (supporting both Illumina and nanopore sequencing), including removal of human DNA and extensive quality control, and runs state-of-the-art ...
Metabarcoding and metagenomics techniques in monitoring airborne pollen
alternative approach is the identification of pollen through molecular sequencing and DNA analysis. Different high-throughput sequencing (HTS) technologies have been used to massively analyse environmental DNA ...
Complete genome reconstruction of the global and European regional dispersal history of the lumpy skin disease virus.
epidemiology involving multiple strains, recombination, and vaccination. Its DNA genome provides limited genetic variation to trace outbreaks in space and time. Sequencing of LSDV whole genomes has also been ...
Complete genome reconstruction of the global and European regional dispersal history of the lumpy skin disease virus.
epidemiology involving multiple strains, recombination, and vaccination. Its DNA genome provides limited genetic variation to trace outbreaks in space and time. Sequencing of LSDV whole genomes has also been ...
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
resolved. Shotgun metagenomics is a sequencing method involving the analysis of the genetic material directly extracted from a sample, without the need for an isolation of the bacteria. By sequencing and ...
Novel prediction models for genotoxicity based on biomarker genes in human HepaRG™ cells.
RNA-sequencing, was investigated. Overall, the RF and SVM models were complementary in their classification of chemicals for genotoxicity. To facilitate data analysis, an online application was developed, combining ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
to the producing GMM if present. We showed that we were able to detect unnatural associations including AMR genes after sequencing all DNA in the samples, confirming the presence of a GMM and ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis. To take the best advantage of long-read sequencing, DNA extraction methods ...
Novel prediction models for genotoxicity based on biomarker genes in human HepaRGTM cells
(NAM s) to identify molecular events underlying the genotoxic mode of action of chemicals. Previously, we developed the GENOMARK biomarker, consisting of 84 genes selected based on whole genomics DNA ...