Electron tomography of negatively stained complex viruses: application in their diagnosis.

Last updated on 22-8-2019 by Anonymous (non vérifié)

Public Access

Published

Peer reviewed scientific article

Anglais

Résumé:

BACKGROUND: Electron tomographic analysis can be combined with the simple and rapid negative staining technique used in electron microscopy based virus diagnosis. METHODS: Standard negative staining of representative examples of parapoxviruses and paramyxoviruses was combined with electron tomographic analysis. RESULTS: Digital sectioning of reconstructions of these viruses at a selected height demonstrated the viral ultrastructure in detail, including the characteristic diagnostic features like the surface threads on C-particles of a parapoxvirus and individual glycoproteins and the …

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