Résultats de la recherche - 174 results
Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR
PCR qPCR real time PCR Real-time PCR Service: Activités transversales en génomique appliquée Transversal activities in applied genomics Transversale activiteiten in toegepaste genomica Category: C32 ...
SYBR®Green qPCR methods for detection of endogenous reference genes in commodity crops: a step ahead in combinatory screening of Genetically Modified Crops in food and feed products
of genetically modified materials imported in the European Union (EU). In order to allow detection of their presence in an integrated screening approach, a set of SYBR-«Green real-time polymerase chain ...
Susceptibility of pigeons to clade 1 and 2.2 high pathogenicity avian influenza H5N1 virus.
detection in real-time reverse transcription PCR (RRT- PCR) and histopathology. At the end of the experiment (14 days PI) blood samples from two pigeons in each group and from contact SPF chickens were also ...
A duplex real-time RT-PCR for the detection of bluetongue virus in bovine semen.
https://www.sciensano.be/en/biblio/a-duplex-real-time-rt-pcr-detection-bluetongue-virus-bovine-semen
(BTV). The aim of this study was to validate a real-time RT- PCR for the direct testing of semen for artificial insemination (AI). The amplification of the BTV target was combined with an internal control ...
Limited genetic diversity and gene expression differences between egg- and non-egg-related Salmonella Enteritidis strains36855
mRNA expression levels of fimA and agfA under conditions of growth simulating the conditions during egg formation were determined by real-time RT- PCR. Although differences in fimA and agfA expression ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
insect-borne disease of domestic and wild ruminants that requires strict monitoring by sensitive, reproducible and robust methods. Real-time reverse transcription polymerase chain reaction (RT-qPCR) analysis has ...
Temperature sensitivity on growth and/or replication of H1N1, H1N2 and H3N2 influenza A viruses isolated from pigs and birds in mammalian cells.
real-time PCR. Multiplication of avian viruses was cold-sensitive, whatever cell type. By contrast, temperature sensitivity of swine viruses was found to depend on the virus and the host cell: for an H1N1 ...
Direct detection and genotyping of Toxoplasma gondii in meat samples using magnetic capture and PCR36884
Quantitative real-time PCR real time PCR Real-time PCR Research Research Support result results Sample Samples SB- IM Sheep Strategies Strategy study Swine The Netherlands Toxoplasma Transmission Type Abstract: ...
Use of pJANUS-02-001 as a calibrator plasmid for Roundup Ready soybean event GTS-40-3-2 detection: an interlaboratory trial assessment.
constructed. The dynamic range, efficiency and limit of detection for the soybean event GTS-40-3-2 real-time quantitative polymerase chain reaction (Q- PCR) system described by Terry et al. (J AOAC Int ...
Fine quantification of avian influenza H5N1 escape mutant quasispecies populations using mutation-specific real-time PCR.
real-time PCR (RT- PCR) tests for the fine quantification of mutations in a background of wild-type sequences. As a proof-of-concept model, we developed mutation-specific RT- PCR tests to quantify antibody ...