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Résultats de la recherche - 6 results
An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material.
classifier as well as read mapping to selected astroviral reference genomes to assess the semiquantitative representation of astrovirus species mixtures. All participants successfully identified and classified ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
level, were specifically designed for this application and need to be assessed. Results: In this study, with different bacterial cocktail mixes, analyzed as pure or spiked in a synthetic fecal matrix, we ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
analytical and interpretation levels since this requires methodological approaches that can target and detect a specific single nucleotide variation (SNV) introduced into a GE organism. In this study, we ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
species in various concentrations and carrying AMR and/or virulence genes, taking the specific need of each application into account. Moreover, the short read and long read approaches are being compared to ...
A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products
fermentation products on the market is prohibited by European regulations. GMM s are currently screened for through qPCR assays targeting AMR genes and vectors, and then confirmed by targeting known specific GM ...
Retrospective survey of unauthorized genetically modified bacteria harbouring antimicrobial resistance genes in feed additive vitamin B2 commercialized in Belgium: Challenges and solutions
feed additive vitamin B2 products. To this end, two real-time PCR markers specific to this unauthorized genetically modified microorganism (GMM), named UGMV it- B2 and 558, were used. In the present ...