Résultats de la recherche - 25 results
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
must be able to isolate pure high molecular weight (HMW) DNA from complex metagenomics samples, without introducing any bias. New methods released on the market, and protocols developed at the research ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
defined as the study of all genetic material within a sample, using sequencing technologies. Shotgun metagenomics is capable of detecting a wide range of microorganisms in a sample, as well as their ...
Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database
Nancy Roosens Source: Food Chemistry: Molecular Sciences, Volume 4 (2022) Health Topics: Food consumption and food safety Health and environment Consommation et sécurité alimentaires Voedselconsumptie en ...
A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products
Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Food Chemistry: Molecular Sciences, Volume 2 (2021) Keywords: AMR genetically modified microorganism identification long and short read sequencing ...
Development of a genoserotyping system for the identification of Salmonella serotypes
detection of molecular markers or on the analysis of sequencing data, are described in the scientific literature. Based on a critical review of some of these techniques, the Multiplex Oligonucleotide ...
Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.
(2017) Keywords: Air Pollution, Indoor DNA Probes environment Fungi Limit of Detection Nucleic Acid Hybridization Abstract: Considered as a public health problem, indoor fungal contamination is generally ...
Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).
oligonucleotide ligation reaction for detection of the molecular marker, followed by a singleplex PCR for signal amplification and analysis of the MOL- PCR products on a Luminex platform. This last step occurs ...
Designation of pathogenic resistant bacteria in the Sparusaurata sea collected in Tunisia coastlines: Correlation with high performance liquid chromatography-tandem mass spectrometry analysis of antibiotics.
alginolyticus. In this study, we reported the phenotypic and molecular characterization of Vibrio parahaemolyticus and Vibrio alginolyticus strains isolated from wild and farm sea bream (Sparus aurata L.) along ...
Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.
therefore important. To avoid problems linked to culture-dependent protocols, molecular methods are increasingly being proposed as an alternative. Among these molecular methods, the polymerase chain reaction ...
Study of the possible migration risks of food contact materials for children under 3 years
detected based on this library search. In Chapter 4.2, unidentified peaks were further investigated by advanced mass spectrometric techniques, such as GC-(EI) TOF- MS and GC-(APCI) QTOF- MS, to specifically ...