Zoekresultaten - 20 results
Bluetongue sentinel surveillance program and cross-sectional serological survey in cattle in Belgium in 2010-2011.
seemed to have completely disappeared by spring 2010. Therefore, a series of repeated cross-sectional surveys, the BT sentinel surveillance program, based on virus detection in blood samples by means of ...
Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.
efficiency with ease-of-automation. Furthermore, six different FMDV-negative matrices were spiked with a dilution series of FMDV SAT1 ZIM 2⅝9. Compared to cell-culture-spiked viral control samples, no matrix ...
Quantification of foot-and-mouth disease virus transmission rates using published data.
makers and modellers alike. However, such quantified parameters are scarcely available, and recently a series of animal experiments was set up to obtain such data experimentally. In this communication, ...
Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.
dilution series and 546 field samples proved that the sensitivity of the BTV RT-qPCR was not affected whereas the beta-actin reaction was reduced only slightly. Without the use of an internal control, 0.6% ...
Foot-and-mouth disease virus: a first inter-laboratory comparison trial to evaluate virus isolation and RT-PCR detection methods.
inclusion of more negative samples and an extension in the serial dilution range of one or more of the FMD positive sample titration series. Health Topics: Animal health Category: A1 FOS Classification: ...
Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe.
EU3), serum samples from naturally and experimentally BHV1-infected cattle, from vaccinated, and vaccinated-challenged cattle, from uninfected cattle, and a series of serum dilutions. In addition, sets of ...
Cloning and sequencing of wsp encoding gene fragments reveals a diversity of co-infecting Wolbachia strains in Acromyrmex leafcutter ants.
Bacterial Outer Membrane Proteins Cloning, Molecular Phylogeny Sequence Analysis, DNA Wolbachia Abstract: By sequencing part of the wsp gene of a series of clones, we detected an unusually high diversity of ...
Tetraponera ants have gut symbionts related to nitrogen-fixing root-nodule bacteria.
examined the ultrastructure of these bacteria and have amplified, cloned and sequenced ribosomal RNA-encoding genes, showing that the ant pouch contains a series of close relatives of Flavobacteria and ...
Implementation of quality assurance in national foot and mouth disease laboratories, based on the guidelines of the Office International des Epizooties.
end, the OIE Standards Commission published a series of guidelines for the evaluation of laboratory quality and proficiency testing. In 1995, the Food and Agriculture Organization European Commission for ...
Secretion of Yop proteins by Yersiniae.
Yersinia enterocolitica Abstract: Upon incubation at 37 degrees C in the absence of Ca2+ ions, pathogenic strains of the genus Yersinia cease growing and produce large amounts of a series of plasmid-encoded ...