A general approach to identify low-frequency variants within influenza samples collected during routine surveillance.

Last updated on 2-10-2023 by Laura Van Poelvoorde

Peer reviewed scientific article



Article written during project(s) : 


Influenza viruses exhibit considerable diversity between hosts. Additionally, different quasispecies can be found within the same host. High-throughput sequencing technologies can be used to sequence a patient-derived virus population at sufficient depths to identify low-frequency variants (LFV) present in a quasispecies, but many challenges remain for reliable LFV detection because of experimental errors introduced during sample preparation and sequencing. High genomic copy numbers and extensive sequencing depths are required to differentiate false positive from real LFV, especially at low…

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