A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

Last updated on 12-3-2020 by Marie-Alice Fraiture

Public Access


Peer reviewed scientific article



Objective The re-emergence and spread of tropical viruses to new areas has raised a wave of concern worldwide. In order to treat patients at an early stage and prevent the diffusion of an outbreak, early diagnosis, and therefore fast and adequate detection, is needed. To this end, a multiplex reverse transcription real-time polymerase chain reaction TaqMan method was designed to detect Zika (ZIKV) and chikungunya (CHIKV) viruses simultaneously. Methods Two methods targeting different genome segments were selected from the literature for each virus. These were adapted for high genom…

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