Biochemical and immunological characterization of a cpn60.1 knockout mutant of Mycobacterium bovis BCG1265

Last updated on 22-8-2019 by Anonymous (niet gecontroleerd)

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Peer reviewed scientific article


Pathogenic mycobacteria possess two homologous chaperones encoded by cpn60.1 and cpn60.2. Cpn60.2 is essential for survival, providing the basic chaperone function, while Cpn60.1 is not. In the present study we show that inactivation of the Mycobacterium bovis BCG cpn60.1 (Mb3451c) gene does not significantly affect bacterial growth in 7H9 broth, but that this knockout mutant (cpn60.1) forms smaller colonies on solid 7H11 medium than the parental and complemented strains. When growing on Sauton medium, the cpn60.1 mutant exhibits a thinner surface pellicle and is associated with higher cult…

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