Evaluation of convenient pretreatment protocols for RNA virus metagenomics in serum and tissue samples.

Last updated on 22-8-2019 by Anonymous (niet gecontroleerd)

Peer reviewed scientific article

SCIENSANO

Samenvatting:

Viral metagenomic approaches are increasingly being used for viral discovery. Various strategies are applied to enrich viral sequences, but there is often a lack of knowledge about their effective influence on the viral discovery sensitivity. We evaluate some convenient and widely used approaches for RNA virus discovery in clinical samples in order to reveal their sensitivity and potential bias introduced by the enrichment or amplifications steps. An RNA virus was artificially spiked at a fixed titer in serum and lung tissue, respectively, low and high nucleic acid content matrices. For ser…

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