On-line high-performance liquid chromatographic diode array spectrophotometric analysis of steroidal hormones in illegal preparations

Last updated on 23-8-2019 by Anonymous (niet gecontroleerd)

Public Access


Peer reviewed scientific article



J. De Beer


An on-line high-performance liquid chromatographic diode array spectroscopic analytical method for the identification of more than 60 different steroidal compounds is described. For the chromatographic separation, a gradient elution that could distinguish the esterified and non-esterified steroids in the same run on a reversed-phase C18 column, using methanol as modifier, was developed. For both types of compound an internal standard was chosen to establish reproducible relative retention times that could be used as one element of the identification; the second element is the UV spectrum, w…

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