Zoekresultaten - 116 results
Molecular epidemiological analysis of the transboundary transmission of 2003 highly pathogenic avian influenza H7N7 outbreaks between the Netherlands and Belgium.
and Belgium. Although no clear epidemiological links could be identified from the tracing data, the transmission network based on concatenated HA- NA- PB2 sequences supports at least three independent ...
Development, validation and evaluation of added diagnostic value of a q(RT)-PCR for the detection of genotype A strains of small ruminant lentiviruses.
Goats Lentivirus Lentivirus Infections Molecular Diagnostic Techniques Molecular Sequence Data Real-Time Polymerase Chain Reaction RNA, Viral Sensitivity and Specificity Sequence Analysis, DNA Sheep Sheep ...
Development and validation of three Capripoxvirus real-time PCRs for parallel testing.
different analytical and diagnostic parameters need to be evaluated to assure data quality. The implementation of parallel testing using multiple real-time PCR s with similar characteristics can improve ...
Setting up a SPF chicken model for the pathotyping of West Nile virus (WNV) strains.
higher and longer-lasting viremias than older chickens. In all experimental groups, the detection window for viral RNA in feathers lasted up to 14 dpi. Altogether, the data presented in this study show ...
Rapid generation of replication-deficient monovalent and multivalent vaccines for bluetongue virus: protection against virulent virus challenge in cattle and sheep.
protection with a multivalent preparation of six distinct DISC viruses. These data indicate that a very-rapid-response vaccine could be developed based on which serotypes are circulating in the population at ...
Experimental infection of Carrion crows (Corvus corone) with two European West Nile virus (WNV) strains.
reliable avian sentinels for WN surveillance in the United States, there is so far no data available about the susceptibility of their Western European counterparts to WNV. Clinical follow-up and serum, oral ...
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.
of the amplification tag. The observed oligonucleotide annealing bias can be reduced by extending the random oligomer sequence and by in silico combining sequence data from SISPA experiments using ...
Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an ...
Importance of identification and typing of Brucellae from West African cattle: a review.
available literature on strains of Brucella in order to provide a state of art-knowledge on species and biovars reported in cattle from West Africa. A literature search was conducted to identify relevant data ...
Diagnosis of Schmallenberg virus infection in malformed lambs and calves and first indications for virus clearance in the fetus.
degree. The VNT test showed that 95% of the malformed lambs were positive for anti- SBV neutralizing antibodies while this was only the case for 44% of malformed calves. These immunological data suggest ...