Zoekresultaten - 28 results
Metagenomics-based tracing of genetically modified microorganism contaminations in commercial fermentation products
product were detected. Presence of AMR genes in the samples was strongly associated with GMM contamination, emphasizing the potential public health risk. Additionally, a variety of other microbial ...
Development of a shotgun metagenomics workflow for the comprehensive surveillance of biological impurities in vitamin-containing food products
LWT, Volume 231 (2025) Keywords: Antimicrobial resistance genes Food safety monitoring genetically modified microorganism shotgun metagenomics Vitamins Abstract: Commercial vitamin-containing food ...
Physicochemical characterization and genotoxicity assessment of nanomaterials using new approach methodologies
genotoxic or non-genotoxic based on gene expression data. In addition, a high-content method to simultaneously assess several genotoxicity parameters (e.g. ɣH2AX, PH3,…) with the Cytek-Amnis ImageStream ...
Retrospective surveillance of viable Bacillus cereus group contaminations in commercial food and feed vitamin B2 products sold on the Belgian market using whole-genome sequencing
whole-genome sequencing (WGS) and subsequently characterized in terms of sequence type (ST), virulence gene profile, antimicrobial resistance (AMR) gene profile, plasmid content, and phylogenomic relationships. ...
Towards facilitated interpretation of shotgun metagenomics long-read sequencing data analyzed with KMA for the detection of bacterial pathogens and their antimicrobial resistance genes
Publication Type: Peer reviewed scientific article Authors: Mathieu Gand; Navickaite, Indre; Bartsch, Lee-Julia; Grützke, Josephine; Overballe-Petersen, Søren; Rasmussen, Astrid; Otani, Saria; Michelacci, Valeria; Matamoros, Bosco Rodríguez; González-Zorn ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
two different STEC strains, and we could cluster separately the reads corresponding to each strain and perform relatedness (phylogeny), however not all genes harbored in the isolate’s genome could be ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) organisms ...
Report on the feasibility of using Hi-C sequencing for metagenomics to define the context of AMR genes
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Josephine Grützke; Carlus Deneke; Jennie Fischer; Lee Julia Bartsch; Bram Bloemen; Kevin Vanneste; Jolien D'aes; Nancy Roosens; Man ...
LC-MS/MS Validation and Quantification of Cyanotoxins in Algal Food Supplements from the Belgium Market and Their Molecular Origins
out of these samples contained microcystin congeners, of which three exceeded 1 µg g−1, a previously proposed guideline value. Additionally, the mcyE gene was amplified and sequenced in ten products to ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
composing genes such as virulence or antimicrobial resistance (AMR) genes, without prior knowledge, nor the need to isolate. Such approaches could offer efficient and fast solutions to limitations faced by ...