Zoekresultaten - 31 results
Retrospective surveillance of viable Bacillus cereus group contaminations in commercial food and feed vitamin B2 products sold on the Belgian market using whole-genome sequencing
Kevin Vanneste Source: Frontiers in Microbiology, Volume 14 (2023) Keywords: Bacillus cereus Food Additives riboflavin Surveillance whole-genome sequencing Abstract: Bacillus cereus is a spore-forming ...
Towards facilitated interpretation of shotgun metagenomics long-read sequencing data analyzed with KMA for the detection of bacterial pathogens and their antimicrobial resistance genes
Publication Type: Peer reviewed scientific article Authors: Mathieu Gand; Navickaite, Indre; Bartsch, Lee-Julia; Grützke, Josephine; Overballe-Petersen, Søren; Rasmussen, Astrid; Otani, Saria; Michelacci, Valeria; Matamoros, Bosco Rodríguez; González-Zorn ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
Keywords: DNA extraction HMW DNA Long-read Metagenomics Method evaluation nanopore Abstract: Background: Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) organisms ...
LC-MS/MS Validation and Quantification of Cyanotoxins in Algal Food Supplements from the Belgium Market and Their Molecular Origins
Issue 8 (2022) Keywords: Food Safety microcystin Microcystis sequencing Tandem Mass Spectrometry; BGAS ultra-high performance liquid chromatography Abstract: Food supplements are gaining popularity ...
Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products
taxon-specific method allows the strengthening of the set of screening markers that are targeting key sequences that are frequently found in GM bacteria (AMR genes and shuttle vector), reinforcing control over the ...
Listeria monocytogenes isolates from Cornu aspersum snails: Whole genome-based characterization and host-pathogen interactions in a snail infection model.
sequencing Abstract: Even though Listeria monocytogenes is an extensive-studied foodborne pathogen, genome analysis of isolates from snails that may represent a reservoir of L. monocytogenes are still scarce. ...
Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database
Publication Type: Peer reviewed scientific article Authors: Assia Saltykova; Julien Van Braekel; N. Papazova; Marie-Alice Fraiture; Deforce, Dieter; Kevin Vanneste; Sigrid C.J. De Keersmaecker; Nancy Roosens Source: Food Chemistry: Molecular Sciences, Vol ...
Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a Salmonella food-borne outbreak
conventional analysis based on isolation, serotyping and MLVA (multilocus variable number tandem repeat analysis) comparison, followed by whole-genome sequencing, to confirm the source of the contamination over ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
sequences between the pUB110 shuttle vector and a Bacillus amyloliquefaciens gene coding for a protease, the presence of the GMM was shown. Based on these sequences of interest, a real-time PCR marker was ...