Zoekresultaten - 68 results
Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus
Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; David Lefebvre; Ilse De Leeuw; Steven Van Borm; Kris De Clercq Source: Journal of Virological Methods, Volume 246 (2017) Health Topics: Animal health Santé animale Diergezon ...
Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator).
A Newcastle disease virus (NDV) was isolated in chicken embryonated eggs after detection by real-time reverse transcription- PCR (RRT- PCR) from a captive owl swab. The complete genome sequence of ...
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.
Reaction Reference Standards RNA, Messenger Abstract: Quantitative real-time PCR assays are widely used for the quantification of mRNA within avian experimental samples. Multiple stably-expressed reference ...
A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
p.e0164463 (2016) Keywords: Animals DNA Primers Foot-and-Mouth Disease Virus Humans Real-Time Polymerase Chain Reaction Abstract: Non-specific tail sequences are often added to the 5’-terminus of primers to ...
Clinical problems due to encephalomyocarditis virus infections in two pig herds.
conventional gel-based polymerase chain reaction tests. The real-time PCR test that gave initially negative result was further optimized to avoid false negative results. CONCLUSIONS: Typical lesions are not ...
Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep.
euthanasia. The presence of SBV RNA in serum and organs was measured with quantitative real-time PCR. The appearance and persistence of neutralizing and SBV nucleoprotein (N) isotype specific antibodies was ...
First isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzania.
with the RBT. Three Brucella were isolated from an aborted fetus and associated membranes. Real time PCR (IS711), Bruce-ladder and classical biotyping classified the isolates as B. abortus biovar 3. ...
European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples: The method of extraction is critical for SBV RNA detection in semen.
Bernd Source: J Vet Diagn Invest, Volume 27, Issue 4, p.422-30 (2015) Keywords: Animals Bunyaviridae Infections Cattle Cattle Diseases Europe Orthobunyavirus Real-Time Polymerase Chain Reaction Reverse ...
Detection and Isolation of Swine Influenza A Virus in Spiked Oral Fluid and Samples from Individually Housed, Experimentally Infected Pigs: Potential Role of Porcine Oral Fluid in Active Influenza A Virus Surveillance in Swine.
(2015) Keywords: Animals Chick Embryo Influenza A Virus, H1N1 Subtype Influenza A Virus, H3N2 Subtype Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Saliva Swine ...
Bluetongue virus RNA detection by real-time rt-PCR in post-vaccination samples from cattle.
positive using BTV-specific real-time RT- PCR, raising questions about a possible low level of virus circulation. An interference of the BTV-8 inactivated vaccine on the result of the real-time RT- PCR was ...