Zoekresultaten - 68 results
Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains.
difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed and validated a new duplex real-time PCR capable of ...
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison.
Véronique Duquesne; Severine Matthijs Source: Insects, Volume 13, Issue 1 (2021) Keywords: Aethina tumida Diagnosis honey bee inter-laboratory comparison Morphology Real-time PCR Small Hive Beetle Abstract: ...
Metagenomic sequencing determines complete infectious bronchitis virus (avian Gammacoronavirus) vaccine strain genomes and associated viromes in chicken clinical samples.
segment B closely related to D3976 /1 from Germany, 2017) and avian orthoreovirus, while three avian orthoavulavirus 1 reads confirmed prior real-time RT- PCR result. IBV sequence variation analysis ...
Detection of Clinical and Subclinical Lumpy Skin Disease Using Ear Notch Testing and Skin Biopsies.
subclinical, the sensitivity of clinical surveillance could be lower than expected. Furthermore, real-time PCR for the detection of LSD viral DNA in blood samples from subclinical animals is only intermittently ...
Retrospective survey of unauthorized genetically modified bacteria harbouring antimicrobial resistance genes in feed additive vitamin B2 commercialized in Belgium: Challenges and solutions
feed additive vitamin B2 products. To this end, two real-time PCR markers specific to this unauthorized genetically modified microorganism (GMM), named UGMV it- B2 and 558, were used. In the present ...
Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From Produced and Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer.
protocols used was successful in removing the viral genome completely from the produced and derived embryos as was demonstrated by real-time PCR. Moreover, BTV-8 virus was transmitted to recipient cows after ...
Development and validation of a TaqMan probe-based real-time PCR method for the differentiation of wild type lumpy skin disease virus from vaccine virus strains.
associated with adverse reactions resembling disease symptoms. The latter hampers clinical diagnosis and poses challenges in virus identification. To this end, a duplex quantitative real-time PCR method ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
and Specificity Sheep Swine Abstract: The 3D and 5UTR real-time RT- PCR assays (RT-qPCR) from Callahan et al. (2002) and Reid et al. (2002) are commonly used reference methods for the detection of ...
Brucella abortus is Prevalent in Both Humans and Animals in Bangladesh.
human sera and all animal samples were screened by Brucella genus-specific real-time PCR (RT- PCR), and positive samples were then tested by IS711 RT- PCR to detect B. abortus and B. melitensis DNA. Only ...
Detection and characterization of Brucella spp. in bovine milk in small-scale urban and peri-urban farming in Tajikistan.
milk in the urban and peri-urban area of Dushanbe, Tajikistan. Brucella DNA was detected in 10.3% of 564 cow milk samples by IS711-based real-time PCR. This finding is concerning because consumption of ...