Zoekresultaten - 18 results
Development and validation of a TaqMan probe-based real-time PCR method for the differentiation of wild type lumpy skin disease virus from vaccine virus strains.
associated with adverse reactions resembling disease symptoms. The latter hampers clinical diagnosis and poses challenges in virus identification. To this end, a duplex quantitative real-time PCR method ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
and Specificity Sheep Swine Abstract: The 3D and 5UTR real-time RT- PCR assays (RT-qPCR) from Callahan et al. (2002) and Reid et al. (2002) are commonly used reference methods for the detection of ...
Brucella abortus is Prevalent in Both Humans and Animals in Bangladesh.
human sera and all animal samples were screened by Brucella genus-specific real-time PCR (RT- PCR), and positive samples were then tested by IS711 RT- PCR to detect B. abortus and B. melitensis DNA. Only ...
Detection and characterization of Brucella spp. in bovine milk in small-scale urban and peri-urban farming in Tajikistan.
milk in the urban and peri-urban area of Dushanbe, Tajikistan. Brucella DNA was detected in 10.3% of 564 cow milk samples by IS711-based real-time PCR. This finding is concerning because consumption of ...
Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus
Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; David Lefebvre; Ilse De Leeuw; Steven Van Borm; Kris De Clercq Source: Journal of Virological Methods, Volume 246 (2017) Health Topics: Animal health Santé animale Diergezon ...
Complete Genome Sequence of a Vaccinal Newcastle Disease Virus Strain Isolated from an Owl (Rhinoptynx clamator).
A Newcastle disease virus (NDV) was isolated in chicken embryonated eggs after detection by real-time reverse transcription- PCR (RRT- PCR) from a captive owl swab. The complete genome sequence of ...
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.
Reaction Reference Standards RNA, Messenger Abstract: Quantitative real-time PCR assays are widely used for the quantification of mRNA within avian experimental samples. Multiple stably-expressed reference ...
A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR.
p.e0164463 (2016) Keywords: Animals DNA Primers Foot-and-Mouth Disease Virus Humans Real-Time Polymerase Chain Reaction Abstract: Non-specific tail sequences are often added to the 5’-terminus of primers to ...
Clinical problems due to encephalomyocarditis virus infections in two pig herds.
conventional gel-based polymerase chain reaction tests. The real-time PCR test that gave initially negative result was further optimized to avoid false negative results. CONCLUSIONS: Typical lesions are not ...
Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep.
euthanasia. The presence of SBV RNA in serum and organs was measured with quantitative real-time PCR. The appearance and persistence of neutralizing and SBV nucleoprotein (N) isotype specific antibodies was ...