Zoekresultaten - 22 results
ESCAIDE 2022 Oral presentation: International outbreak of Salmonella Typhimurium linked to a chocolate factory in 2022: Belgian findings
were analysed for Salmonella spp. using real-time PCR. Whole genome sequence (WGS) analysis of isolates of probable cases and positive food samples is still ongoing. Results We identified 62 probable ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
Microbiology, Volume 354 (2021) Keywords: Antimicrobial resistance genes DNA walking anchored on pUB110 food and feed safety Genetically modified bacteria Protease real-time PCR detection Abstract: A PCR ...
Retrospective survey of unauthorized genetically modified bacteria harbouring antimicrobial resistance genes in feed additive vitamin B2 commercialized in Belgium: Challenges and solutions
feed additive vitamin B2 products. To this end, two real-time PCR markers specific to this unauthorized genetically modified microorganism (GMM), named UGMV it- B2 and 558, were used. In the present ...
Development of a genoserotyping system for the identification of Salmonella serotypes
Publication Type: Dissertation Authors: Mathieu Gand Source: Ghent University, Ghent, p.185 (2020) Keywords: Salmonella; Genoserotyping; Luminex; Decision support system; Food safety; real-time pcr ...
Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products
First, the potential presence of the AMR gene is detected by real-time PCR. Next, its full-length is evaluated by a nested- PCR amplifying a large fragment of its sequence to determine the risks of likely ...
A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.
presented results demonstrate the potential of the modified Magnetic Capture real time PCR as a full alternative to the mouse bioassay for the screening of various types of tissues and meat, with the ...
Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.
(PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if these tools have demonstrated their appropriate performance, some of them are not able to ...
Erratum to: Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
Publication Type: Peer reviewed scientific article Authors: Libert, X; Camille Chasseur; Bladt, S; Ann Packeu; Bureau, F; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Appl Microbiol Biotechnol, Volume 99, Issue 19, p.8319-20 (2015) Health Topics: F ...
Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
excellent alternative for the monitoring of indoor fungal contaminations. As a case study, we developed a SYBR ® green real-time PCR-based assay for the specific detection and identification of Aspergillus ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing
number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...