Zoekresultaten - 71 results

An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen.

a good substitute for the slow and laborious virus isolation, for the screening testing at artificial insemination centres and for international trade. Health Topics:  Animal health Category:  A1 FOS ...

Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen.

conventional virus isolation method for the detection of BoHV-1 in extended semen. The specificity of the assay is as expected. The assay had an analytical sensitivity of 0.38 TCID (50) virus spiked into ...

A universal avian endogenous real-time reverse transcriptase-polymerase chain reaction control and its application to avian influenza diagnosis and quantification.

a universal avian endogenous internal control (bird beta-actin) and apply it to influenza A diagnosis. Moreover, this internal control proves useful as a normalizer control for virus quantification, because ...

Lethality and molecular characterization of an HPAI H5N1 virus isolated from eagles smuggled from Thailand into Europe.

nipalensis, smuggled into Europe from Thailand were seized at Brussels International Airport. A highly pathogenic avian influenza virus, denominated A/crested eagle/Belgium/0½004, was isolated from these birds ...

Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments.

Keywords: Animals Bluetongue Bluetongue virus Cell Line Cricetinae DNA Primers DNA Probes Genome, Viral Nucleic Acid Amplification Techniques Reproducibility of Results Reverse Transcriptase Polymerase Chain ...

Avian influenza: mini-review, European control measures and current situation in Asia.

contribute to the dissemination of the virus. In the context of H5N1, all movements (trade, high international mobility, migration and smuggling) can become high risk factors of spreading the disease. In most ...

Binding and entry characteristics of porcine circovirus 2 in cells of the porcine monocytic line 3D4/31.

/31 were studied by visualization of binding and internalization of PCV2 virus-like particles (VLP s) by confocal microscopy and chemical inhibition of endocytic pathways (clathrin- and ...

Integrating adherence to highly active antiretroviral therapy into children's daily lives: a qualitative study.

adherence. Adherent patients were found to internalize the medical information to a stronger extent than less-adherent patients. Adherent patients showed stronger motivation to stick to the medical regimen on ...

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