Zoekresultaten - 8 results
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
Ultimately, a validation of the method is still necessary in order to obtain a precise limit of detection based on the analysis of a large dataset of samples. Moreover, other technologies can still be ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis. To take the best advantage of long-read sequencing, DNA extraction methods ...
Simultaneous Detection and Quantification of Aflatoxin M1, Eight Microcystin Congeners and Nodularin in Dairy Milk by LC-MS/MS
extracted with methanol 80%, followed by an SPE clean-up step before LC- MS / MS analysis. The LOQ was fixed at 1 µg/L for the nine cyanotoxins and 0.05 µg/L for AFM1. Recoveries were measured between 82.67% ...
Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Mathieu Gand; Indre Navickaite; Josephine Gruetzke; Lee-Julia Bartsch; Saria Otani; Giuliano Garofolo; Marco Di Domenico; Valeria Michel ...
Optimised long-read sequence bioinformatics tool for the analysis of the resistome and microbial communities
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Manal AbuOun; Saria Otani; Indre Navickaite; Mathieu Gand; Bram Bloemen; Kevin Vanneste; Valeria Michelacci; Bosco Rodríguez Matamoros; Nancy Roosens; Sigrid C.J. De ...
Nanomaterials for food applications: Analytical challenges and practical solutions for enforcing labeling of nanoingredients in food products in the European Union
food products in the European Union, Elsevier (2019) ISBN: 978-0-12-814130-4 Keywords: analysis electron microscopy Field-flow fractionation food Food Additives Ingredients labeling nanomaterials ...
Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.
Besides the technological advances which have pushed the approach forward, the last years have been marked by considerable evolution of the whole genome sequencing data analysis methods. Prior to ...
In vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
methyl ester whereas others ask an in-depth analysis to confirm their activity. For active substances only the study will be continued and a full dose-response curve will be performed in order to assess ...