Zoekresultaten - 13 results
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
Keywords: DNA extraction HMW DNA Long-read Metagenomics Method evaluation nanopore Abstract: Background: Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which ...
Simultaneous Detection and Quantification of Aflatoxin M1, Eight Microcystin Congeners and Nodularin in Dairy Milk by LC-MS/MS
Keywords: cyanotoxins; aflatoxin M1 dairy milk Food Safety liquid chromatography–mass spectrometry method development Abstract: Dairy milk holds a prominent position as a widely consumed food, particularly ...
Residential Exposure to Urban Trees and Medication Sales for Mood Disorders and Cardiovascular Disease in Brussels, Belgium: An Ecological Study
prescribed for mood disorders and cardiovascular disease. Methods: We developed a workflow to automatically isolate all individual trees from airborne light detection and ranging (LiDAR) data collected in ...
In utero exposure to parabens and early childhood BMI z-scores – Associations between placental ethyl paraben, longitudinal BMI trajectories and cord blood metabolic biomarkers
investigated longitudinal effects of in utero paraben exposure on early childhood trajectories of BMI z-scores. Methods: Placental concentrations of four parabens [methyl (MeP), ethyl (EtP), propyl (PrP), and ...
Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants
Keersmaecker Source: Genes, Volume 12, Issue 4 (2021) Keywords: SARS-CoV-2; COVID-19; variants; detection; RT-qPCR; in silico specificity evaluation; bioinformatics tool; WGS data; mismatches; primers and probes ...
Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.
(consisting of a total of 30 primers and probe sets) used for SARS-CoV-2 detection and the impact of the virus’ genetic evolution on four of them. The exclusivity of these methods was also assessed using the ...
Evaluation of a TEM based Approach for Size Measurement of Particulate (Nano)materials
Source: Materials, Volume 12, Issue 14 (2019) Keywords: electron microscopy European Commission’s Recommendation for a definition of a nanomaterial image analysis Method validation nanomaterial particulate ...
Simultaneous determination of parabens, bisphenols and alkylphenols in human placenta by ultra-high performance liquid chromatography- tandem mass spectrometry
limits of detection and quantification are in the range of 0.1-0.3 ng g−1 and 0.2-0.7 ng g−1, respectively. The applicability of the method was demonstrated on 71 human placenta samples from a Belgian ...
A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay
microscopic identification which are complex, sometimes ambiguous and time-demanding, i.e., up to 21 days. Therefore, molecular, culture-independent methods could be more advantageous for the monitoring of E. ...
Molecular detection and genotyping of noroviruses
journal Laboratories LEVEL levels low level matrix method methods microbiology Molecular molecular detection ON polymerase chain reaction preservation problems public public health Public-health ...