Zoekresultaten - 10 results

Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.

Clinical Laboratory Techniques DNA Primers DNA, Viral European Union Real-Time Polymerase Chain Reaction Reference Standards Reproducibility of Results Sensitivity and Specificity Swine Veterinary Medicine ...

Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.

(RT-qPCR) was used routinely for laboratory diagnosis during the 2006/2007 bluetongue virus (BTV) serotype 8 epidemic. In the present study the impact of pooling and multiplexing strategies on RT-qPCR are ...

Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus.

Classical Swine Fever (CSF) National Reference Laboratory. The producers were asked to introduce an internal control to check the RNA extraction efficacy. The different criteria assessed were sensitivity, ...

Real-time laboratory exercises to test contingency plans for classical swine fever: experiences from two national laboratories.

Swine Fever Cooperative Behavior denmark Diagnosis, Differential Disaster Planning Disease Outbreaks Female Humans International Agencies Laboratories Male Abstract: In order to adequately and efficiently ...

Foot-and-mouth disease virus: a first inter-laboratory comparison trial to evaluate virus isolation and RT-PCR detection methods.

the initial suspensions. Each laboratory tested the samples by one or more of its available RT- PCR procedures and inoculated cell cultures that it routinely uses for FMD diagnosis in attempts to ...

Analytical performance of several classical swine fever laboratory diagnostic techniques on live animals for detection of infection.

p.137-43 (2004) Keywords: Animals Antigens, Viral Classical Swine Fever Classical swine fever virus Clinical Laboratory Techniques Enzyme-Linked Immunosorbent Assay Reverse Transcriptase Polymerase Chain ...

Diagnosis of foot-and-mouth disease by RT-PCR: evaluation of primers for serotypic characterisation of viral RNA in clinical samples.

virus by reverse transcription polymerase chain reaction (RT- PCR) at the OIE / FAO World Reference Laboratory for FMD (WRL), Pirbright. The primers had been characterised previously elsewhere on ...

Laboratory activities involving transmissible spongiform encephalopathy causing agents: risk assessment and biosafety recommendations in Belgium.

stopping TSE diagnosis due to a decreasing number of TSE cases reported in the last years, decontamination procedures and a protocol for decommissioning TSE diagnostic laboratories is proposed. Health ...

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