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Zoekresultaten - 12 results
Development of a multiplex-based immunoassay for the characterization of diphtheria, tetanus and acellular pertussis antigens in human combined DTaP vaccines.
focused on the development of a Luminex-based multiplex assay to monitor the consistency of antigen quantity and quality throughout the production process of DT aP vaccines from two human vaccine ...
Development and validation of a microfluidic multiplex immunoassay for the determination of levels and avidity of serum antibodies to tetanus, diphtheria and pertussis antigens.
Methods, Volume 503 (2022) Keywords: Antibodies, Bacterial Bordetella pertussis Diphtheria Humans Immunoassay Immunoglobulin G Microfluidics Pertussis Toxin Tetanus Whooping Cough Abstract: A multiplex assay ...
Development of a multiplex immunoassay for simultaneous detection of antigens in DTaP vaccines
that could be implemented for routine potency assessment of vaccines. Our work focused on the development of a Luminex-based multiplex assay (four to six-plex assay depending on the number of pertussis ...
A molecular assay for rapidly distinguishing the AviPro SALMONELLA VAC T vaccine strain from wild-type field isolates
Volume 184 (2021) Keywords: Luminex-based multiplex test Molecular differentiation Salmonella typhimurium Vac T vaccin Abstract: Rapid differentiation of the AviPro Salmonella VAC T strain from ...
Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay.
(e.g. virus neutralization assays), or are expensive with suboptimal specificity (e.g. commercial ELISA s and RDT s). Bead-based assays offer a cost-effective alternative and allow for multiplexing to ...
Development of a genoserotyping system for the identification of Salmonella serotypes
detection of molecular markers or on the analysis of sequencing data, are described in the scientific literature. Based on a critical review of some of these techniques, the Multiplex Oligonucleotide ...
Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.
misidentified E. coli samples at the National Reference Centre (NRC). In this paper, we describe two novel tools to enhance Shigella surveillance. First, we developed a low-cost Luminex-based multiplex assay ...
RNA-based drug susceptibility testing of Mycobacterium tuberculosis
performed directly on crude cell lysate using a combination of the bead-based MagPixTM (Luminex) and QuantigeneTM Plex (Thermo Fischer) technology. Normalized, relative genes expression levels (control vs ...
Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
issues. The multiplexing issue was overcome through a Luminex xMAP® assay, developed for the simultaneous detection of the 10 most frequently in indoor air found fungi. All the species identified with the ...
Optimized MOL-PCR for Characterization of Microbial Pathogens
surveillance, outbreak detection, and tracing of outbreak sources. This unit describes a multiplex oligonucleotide ligation- PCR (MOL- PCR) optimized for characterization of microbial pathogens. With MOL- PCR, ...