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Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
water, the DNA was extracted with two classical commercial kits or with a kit performing depletion of the host DNA. The extracted DNA also was amplified or not using Phi29 polymerase. We showed that all ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
evaluated the performances of 6 DNA extraction methods using various cells lysis and purification techniques, from quick and easy, to more time-consuming and gentle protocols, including a portable method for ...
Simultaneous determination of parabens, bisphenols and alkylphenols in human placenta by ultra-high performance liquid chromatography- tandem mass spectrometry
1121, Number 102 (2019) Keywords: Alkylphenol Bisphenol endocrine disruptor Paraben Placenta UHPLC-MS/MS Abstract: This study presents de development and validation of an ultra- high performance liquid ...
Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.
PLoS One, Volume 13, Issue 2 (2018) Keywords: bacterial phylogeny; food-borne; genes; NGS; WGS; computational biology/bioinformatics; disease outbreaks; outbreak; performance evaluation; pipeline; ...