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Zoekresultaten - 6 results
Characterization of new Alternaria alternata-specific rat monoclonal antibodies
A1-based sandwich ELISA efficiently detected this epitope in various mould (but not yeast)-soluble extracts prepared from strains grown in the laboratory. Moreover, this A1-based sandwich ELISA detected its ...
Estimation of the dissociation rate of unlabelled ligand-receptor complexes by a 'two-step' competition binding approach.
APPROACH: We used the ‘two-step competition’ binding approach consisting of pre-incubating the receptor preparation with a wide range of ligand concentrations, washing off free ligand molecules, adding ...
Evidence for the presence of Legionella bacteriophages in environmental water samples.
were isolated from various fresh water environments, and preliminary characterization showed that these crude preparations infect exclusively bacteria belonging to the genus Legionella. Standard phage ...
Antagonist-radioligand binding to D2L-receptors in intact cells.
were predominantly done with broken cell preparations with the tritiated D(2)-receptor antagonists [(3)H]-raclopride, a hydrophilic benzamide, and [(3)H]-spiperone, a highly hydrophobic butyrophenone. ...
Culture filtrate specific H-2(b) restricted CD8+ T cells activated in vivo by Mycobacterium tuberculosis or bovis BCG recognize a restricted number of immunodominant peptides.
antigen presenting cells. MHC class I bound peptides were acid eluted from the surface of RMA-S cells incubated with M. tuberculosis culture filtrate proteins. The crude peptide preparation was able to ...
Induction of immunity by DNA vaccination: application to influenza and tuberculosis.
tuberculosis antigen (antigen 85; Ag85) were prepared and tested as DNA vaccines in mice. In both cases, high titer antibody responses and robust cell-mediated immune responses were induced against the ...