Zoekresultaten - 23 results

A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products

fermentation products on the market is prohibited by European regulations. GMM s are currently screened for through qPCR assays targeting AMR genes and vectors, and then confirmed by targeting known specific GM ...

How can we better detect unauthorized GMO in the food and feed chain

6, Number 517 (2017) Keywords: detection DNA walking Monitoring new workflow next-generation-sequencing qPCR unauthorized GMO Abstract: Current GMO detection systems have limited abilities to detect ...

An integrated strategy combining DNA walking and NGS to detect GMO

Volume 232, Number 358 (2017) Keywords: detection DNA walking GMO Next-generation sequencing qPCR Abstract: Recently, we developed a DNA walking system for the detection and characterization of a broad ...

Biotech Rice: Current developments and future detection challenges in food and feed chain

choice, regulatory frameworks were established in many countries around the world, such as in Europe. Their implementations, including detection systems usually based on qPCR, are becoming complex and ...

Current and new approaches in GMO detection: challenges and solutions

DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. ...

Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.

with qPCR screening, usually used in GMO routine analysis, as well as being able to detect, characterize and identify a broad spectrum of GMO s in food/feed matrices, two bidirectional DNA walking ...

Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing

number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...

New trait-specific qualitative SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS

European Food Research and Technology, Volume 241, Issue 2 (2015) Keywords: New qualitative trait-specific SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS Abstract: ...

Validation of a sensitive DNA walking strategy to characterise unauthorised GMOs using model food matrices mimicking common rice products

integrated approach has recently been developed targeting pCAMBIA family vectors, highly present in transgenic plants. Their presence is first assessed by qPCR screening and is subsequently confirmed by ...

Validation of the entire CoSYPS Path Food workflow for qPCR detection, identification, isolation and confirmation of food-borne pathogenic bacteria present in carcass samples

(2014) Keywords: bacteria CoSYPS CoSYPS,Salmonella,Listeria,qPCR,entire workflow validation detection Europe food identification method methods pathogenic qPCR Sample Samples VALIDATION Health Topics:  ...

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