Zoekresultaten - 7 results
Introduction of WGS in food microbiology: advantages and challenges
prepared using a self-tasking mandate and published after public consultation. The added value of WGS for the detection of foodborne outbreaks and bacterial risk assessment has been demonstrated on many ...
Comparison of Six Chromogenic Agar Media for the Isolation of a Broad Variety of Non-O157 Shigatoxin-Producing Escherichia coli (STEC) Serogroups.
strains were used, including 39 STEC strains belonging to 16 different O serogroups and 6 non- STEC E. coli. All E. coli strains were able to grow on TBX and RB, whereas one STEC strain was unable to grow ...
Toxoplasma gondii and Neospora caninum in wildlife: common parasites in Belgian foxes and Cervidae?
from Cervidae were tested for the presence of antibodies against Neospora caninum using ELISA; and against Toxoplasma gondii using SAG1- ELISA and a commercially available agglutination test. The T. ...
Prioritisation of food-borne parasites in Europe, 2016.
to rank food-borne parasites (FBP) in terms of their importance for Europe and regions within Europe. Countries were divided into European regions according to those used by the European Society of ...
Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces.
genes stx1 and stx2 and the intimin gene eae quantification was optimized for ddPCR analysis. The properties of ddPCR and qPCR using two different mastermixes (EMM: TaqMan(®) Environmental Master Mix 2.0; ...
RNA sequencing-based transcriptional overview of xerotolerance in Cronobacter sakazakii SP291.
formula, often resulting in high mortality rates. Here, we used transcriptome sequencing (RNA-seq) to provide transcriptional insights into the survival of C. sakazakii in desiccated conditions. Our ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.
especially when using Nextera XT library preparation. This study contributes to addressing the WGS challenges of standardizing protocols to support data portability and to enable full exploitation of ...