Strategy for the identification of micro-organisms producing food and feed products: Bacteria producing food enzymes as study case

Last updated on 12-3-2020 by Marie-Alice Fraiture

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Peer reviewed scientific article

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Recent European regulations require safety assessments of food enzymes (FE) before their commercialization. FE are mainly produced by micro-organisms, whose viable strains nor associated DNA can be present in the final products. Currently, no strategy targeting such impurities exists in enforcement laboratories. Therefore, a generic strategy of first line screening was developed to detect and identify, through PCR amplification and sequencing of the 16S-rRNA gene, the potential presence of FE producing bacteria in FE preparations. First, the specificity was verified using all microbial spec…

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