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Zoekresultaten - 13 results
Attributing Ethiopian animal health losses to high-level causes using expert elicitation
class, and production system. Three-point questions were used to inform beta-pert distributions and capture uncertainty in estimates. Individual expert estimates were aggregated by quantile mean to produce ...
Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf
Antimicrobial resistance genes acting against aminoglycosides, beta-lactam antibiotics, fosfomycin, quinolones, sulphonamides, trimethoprim and tetracyclines were found. The calf recovered after empirical ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
purposes, both assays were run in parallel in a triplex one-step RT-qPCR protocol with beta-actin as an internal control and synthetic RNA as an external control. We obtained low limits of detection and high ...
Prediction of respiratory disease and diarrhea in veal calves based on immunoglobulin levels and the serostatus for respiratory pathogens measured at arrival.
globulins, beta-globulins and Ig’s) at arrival and BRD and NCD in the first 3 weeks of the production cycle. The second objective was to determine whether the serostatus (seropositive/seronegative) of seven ...
Study of the virulence of serotypes 4 and 9 of African horse sickness virus in IFNAR(-/-), Balb/C and 129 Sv/Ev mice.
Interferon-alpha mice Mice, Inbred BALB C Mice, Knockout Receptor, Interferon alpha-beta RNA, Double-Stranded Serogroup virulence Abstract: African horse sickness virus (AHSV) is a double-stranded RNA virus which ...
Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.
virology Abstract: A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous ...
Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression.
(2010) Keywords: Animals Antibodies, Monoclonal Antigens, CD45 Blood Platelets Cell Separation Chickens Erythrocytes Flow Cytometry Interleukin-1beta Interleukin-6 Leukocytes Phycoerythrin Staining and ...
Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.
workload is to apply a multiplexing strategy in which the viral RNA and internal beta-actin control RNA are detected in a single reaction. Parallel analysis (singleplex versus multiplex) of a 10-fold ...
A universal avian endogenous real-time reverse transcriptase-polymerase chain reaction control and its application to avian influenza diagnosis and quantification.
avian endogenous internal control (bird beta-actin) and apply it to influenza A diagnosis. Moreover, this internal control proves useful as a normalizer control for virus quantification, because ...
Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments.
strains. Therefore these two methods are complementary and could be used in parallel to confirm the diagnosis of a possible new introduction of BTV. An RT-qPCR amplifying a fragment of the beta-actin mRNA ...