Zoekresultaten - 72 results
Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
versicolor, which is frequently observed in indoor environment and known to be allergenic. The developed primers amplify a short region of the internal transcribed spacer 1 from the 18S ribosomal DNA complex. ...
Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.
p.790170 (2015) Keywords: DNA Primers DNA, Bacterial Humans Microspheres Multiplex Polymerase Chain Reaction Oligonucleotide Probes Salmonella typhimurium Abstract: With multiplex oligonucleotide ligation- ...
The RNA template channel of the RNA-dependent RNA polymerase as a target for development of antiviral therapy of multiple genera within a virus family.
template-primer duplex. Elucidation of a crystal structure of the inhibitor bound to CVB3 3Dpol confirmed the RNA-binding channel as the target for GPC- N114. Docking studies of the compound into the crystal ...
A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test.
two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT- PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species. Primers with 17 to ...
SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.
Issue 22, p.9811-24 (2013) Keywords: Bacterial Proteins Bacteriological Techniques DNA Primers Fluorescent Dyes Organic Chemicals Real-Time Polymerase Chain Reaction Reproducibility of Results Salmonella ...
An innovative and integrated approach based on DNA walking to identify unauthorised GMOs
cassette and the rice genome. To this end, a DNA walking strategy is applied using a first reverse primer followed by two semi-nested PCR rounds using primers that are each time nested to the previous ...
An innovative integrated approach based on DNA walking to identify unauthorized GMOs
of the junction between the transgenic cassette and the rice genome. To this end, a DNA walking strategy is applied using a first reverse primer followed by two semi-nested PCR rounds using primers ...
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.
Volume 8, Issue 9, p.e76144 (2013) Keywords: Avulavirus bias DNA Primers DNA, Complementary High-Throughput Nucleotide Sequencing Nucleic Acid Amplification Techniques virology Abstract: Sequence ...
Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.
of Listeria spp. The selected primers were designed to amplify short fragments (60 to 103 bp) in order to obtain optimal PCR efficiency (between 97 and 107 % efficiency). The limit of detection of the ...
Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology
types of Luminex assays can be envisaged. Each assay has its advantages and limitations, some of which are related to the inherent design of the primers and probes used. Software that predicts melting ...