Zoekresultaten - 7 results

Introduction of WGS in food microbiology: advantages and challenges

for the Safety of the Food Chain (FASFC) published the opinion 18-2021 regarding whole genome sequencing (WGS) for the detection of foodborne outbreaks and bacterial risk assessment. This opinion was ...

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies

concept using Shiga toxin-producing Escherichia coli spiked food samples for a strain-level metagenomics foodborne outbreak investigation method using the MinION and Flongle flow cells from Oxford Nanopore ...

Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks

of S. aureus isolates, including some belonging to a confirmed foodborne outbreak, its added value as the ultimate multiplexing method was demonstrated. In contrast to PCR-based se gene detection for ...

Staphylococcal enterotoxin gene cluster: prediction of enterotoxin (SEG and SEI) production and of the source of food poisoning based on Saβ typing.

many people. Most of these outbreaks remain undiscovered as detection methods for enterotoxins are only available for a few enterotoxins, but not for the more recently discovered enterotoxin G (SEG) and ...

NAuRA: Genomic Tool to Identify Staphylococcal Enterotoxins in Strains Responsible for FoodBorne Outbreaks.

SE s constitute a protein family with 27 proteins. However, enzyme immunoassays can only detect directly in food the five classical SE s (SEA- SEE). Thus, molecular characterization methods of strains ...

A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

to support EC legislation. As part of this mandated work programme, merging of ISO / TS 21872-1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ...

Food-Borne Outbreak Investigation and Molecular Typing: High Diversity of Staphylococcus aureus Strains and Importance of Toxin Detection

and in healthy persons carrying enterotoxin genes encoding emetic SE s for which no detection methods currently exist. For one outbreak, the investigation pointed out to the food handler who transmitted ...

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