Zoekresultaten - 25 results
Development of a reliable UHPLC-MS/MS method for simultaneous determination of zearalenone and zearalenone-14-glucoside in various feed products
thoroughly validated in terms of linearity (R2 ≥ 0.999), sensitivity (limit of quantification in the range of 0.50–5.00 μg kg−1), recovery (89.35 ± 2.67% to 110.93 ± 1.56%), and precision (RSD, 3.00–14.20%), ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
taxonomic classification up to an unprecedented sensitivity, including the identification of linked specific genes, such as AMR or virulence genes. We are developing and optimizing the protocols (from DNA ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
developed to target specifically the newly discovered GMM, namely GMM protease2. The performance of the real-time PCR marker was assessed in terms of specificity and sensitivity. The applicability of the ...
Strategy for the identification of micro-organisms producing food and feed products: Bacteria producing food enzymes as study case
amplification and sequencing of the 16S-rRNA gene, the potential presence of FE producing bacteria in FE preparations. First, the specificity was verified using all microbial species reported to produce FE. ...
Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.
isolates and sensitivity towards sequencing coverage, which could be linked to the specific data analysis strategies used therein. To demonstrate the effect of particular data analysis steps, several ...
A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.
cyst density, those have proved to be insufficiently sensitive. A major improvement of the sensitivity was achieved with magnetic capture-based DNA extraction. By combining the hybridization of specific ...
Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Combined Species Identification and Drug Sensitivity Testing in Mycobacteria.
Combined species identification was performed using the Biotyper Mycobacteria Library 4.0. Mycobacterium-specific MBT- ASTRA parameters were derived (calculation window, m/z 5,000 to 13,000, area under the ...
Study of the possible migration risks of food contact materials for children under 3 years
a selection of representative baby bottles. For migration testing, the use of simulants is prescribed in the legislation to mimic the testing of real foods. Specifically, a mixture of water-EtOH (50:50, v/v) is ...
Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.
Reproducibility of Results Sensitivity and Specificity Staining and Labeling Abstract: Currently, contamination of indoor environment by fungi and molds is considered as a public health problem. The monitoring of ...
New trait-specific qualitative SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS
Method acceptance parameters such as the sensitivity, specificity and repeatability were assessed as well as the robustness of the methods. Additionally, the reproducibility was evaluated by transferring ...