Zoekresultaten - 80 results
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
adaptive sampling, following whole genome amplification to increase the amount of genetic material to meet sequencing standards. The strain-level data analysis was performed as previously described (Buytaers ...
Belgian Mosquitoes Are Competent for Japanese Encephalitis Virus and Readily Feed on Pigs, Suggesting a High Vectorial Capacity.
flavivirus which uses pigs as amplification hosts, we investigated (1) whether would feed on pigs and (2) its vector competence for JEV, to investigate whether this species could be a potential vector. Three- ...
Occurrence of microcystin congeners in surface waters and food in Belgium as a source of exposure to humans
the producers of these toxins by amplification and sequencing of the mcyE gene. Yet, data on the MC s prevalence is not sufficient to accurately assess human exposure and risk. Additional consumption ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
chosen for further studies (i.e. 24 hours enrichment as stated in the current international standard procedure, classical commercial DNA extraction and no amplification). Cheese samples were spiked with ...
Redesign and Validation of a Real-Time RT-PCR to Improve Surveillance for Avian Influenza Viruses of the H9 Subtype.
assessed according to the OIE standards. To facilitate assay deployment, amplification conditions were optimized with different nucleic extraction systems and amplification kits. Performance of the new ...
A Summer of Cyanobacterial Blooms in Belgian Waterbodies: Microcystin Quantification and Molecular Characterizations
method allowed to detect and quantify eight microcystin congeners. The mcyE gene was detected using PCR, while dominant cyanobacterial species were identified using 16S RNA amplification and direct ...
Efficient control of Japanese encephalitis virus in the central nervous system of infected pigs occurs in the absence of a pronounced inflammatory immune response.
nervous system (CNS), resulting in a detrimental outcome. Pigs act as important amplification and reservoir hosts, and JEV infection of pigs is mostly subclinical. Information on virus spread in the CNS and ...
Development of a genoserotyping system for the identification of Salmonella serotypes
biochemical test. From this molecular markers’ selection, 4 MOL- PCR assays were developed, i.e. the molecular markers were recognized by probes through a ligation-amplification reaction (MOL- PCR), followed by ...
Strategy for the identification of micro-organisms producing food and feed products: Bacteria producing food enzymes as study case
amplification and sequencing of the 16S-rRNA gene, the potential presence of FE producing bacteria in FE preparations. First, the specificity was verified using all microbial species reported to produce FE. ...
Detecting cervical precancer and reaching underscreened women by using HPV testing on self samples: updated meta-analyses.
0.97 to 1.02). However, hrHPV assays based on signal amplification were less sensitive on self samples (pooled ratio 0.85, 95% confidence interval 0.80 to 0.89). The specificity to exclude CIN2 + was 2% ...